1. New Customers - Request a Price Quote, Click Here!
Enter your contact information in the fields provided and specify in the "comments" box that you would like a sequencing quote. Click <Send>
Or call 1-800-RETROGEN.
2. Order Sequencing Online, Click Here!
>>Click Here to download the instructions on how to order online.
3. Order Sequencing by Order Forms
In the "Get Order Forms" menu at the top of page, select the order form for 40 or 96 samples.
Fill out the sequencing order form.
Be sure to include the quote number, P.O. or credit card number, and an email address.
Email your order form to sequencing@retrogen.com
4. Sample Submission Requirements
For detailed information on our template requirements, Click Here!
The primers may be mixed with the template or sent in separate tubes.
Recommended DNA Template and Primer Quantities for Each Reaction
DNA and Primer Separated
DNA |
Concentration |
Amount |
2x Amount |
PCR product DNA |
5-20ng/µl |
50ng |
100ng |
Single stranded plasmid |
50-100ng/µl |
250ng |
500ng |
Double stranded plasmid |
50-200ng/µl |
500ng |
1000ng |
BAC |
100-200ng/µl |
2000ng |
4000ng |
Primer |
10pmol/µl |
5µl |
10µl |
DNA and Primer Combined
DNA |
DNA |
Primer |
Total Vol. |
2X DNA |
2X Primer |
2X Total Vol. |
PCR product DNA |
50ng |
20pmoles |
10uL |
100ng |
40pmoles |
20uL |
Single stranded plasmid |
250ng |
20pmoles |
10uL |
500ng |
40pmoles |
20uL |
Double stranded plasmid |
500ng |
20pmoles |
10uL |
1000ng |
40pmoles |
20uL |
BAC |
2000ng |
20pmoles |
10uL |
4000ng |
40pmoles |
20uL |
We recommend 2x amount of DNA in case we need to repeat the reactions.
Single Tube Sequencing
Smaller orders should use 1.5mL epi tubes. Screw cap tubes are more time consuming so pop caps should be used. Each tube must be clearly labeled to match with the order forms. Tube strips while convenient are hard to label and keep track of so for large orders (24+ samples) use 96 well plates. We recommend using strip lids to cap plates as they prevent leakage and cross contamination – especially for plates shipped overnight.
96 Well Plate sequencing
Please submit your DNA and primers at the concentration according to the table above. The concentration of DNA and primers should be normalized and arrayed vertically from A1-H1, A2-H2…etc. on the 96 well plate. The plate should be sealed or capped using strip lids to prevent leakage and shipped overnight.
Send your samples and a printout of your order form to:
Retrogen, Inc.
Sequencing Dept.
6645 Nancy Ridge Dr
San Diego , CA 92121
We recommend you use overnight delivery service at ambient temperature.
Note: It is not necessary to ship samples in dry ice. The extra weight will only add to your shipping cost.
5. Retrieve Your Sequencing Results
In most cases, sequencing results are available the day after we receive your order. You will receive an email notification. If it is your first order, you will receive a user name and password.
Llogin to our dnaLIMS system, Click Here!
While logged into our dnaLIMS system, you may do the following:
- View results
- Change your password
- Request a pick up ( San Diego customers)
- Enter sequencing orders
6. View Your Sequencing Results, Click Here!
