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---W
e l c o m e t o R e t r o g e n--- |
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New
Customers Receive
2
Free Reactions on the First Sequencing
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Retrovirus
Kit
Retrogen retroviral vectors are Moloney Murine
Leukemia Virus (MoMuLV)-based vectors. We
have constructed a foundation vector, pRT-X,
which has been trimmed to contain the minimal
amount of retroviral sequence required to
produce an infectious, replication deficient,
retrovirus.
A minimized retroviral vector allows
the insertion of larger cDNAs of interest. All vectors contain the CMV promoter/enhancer
fused to a minimal fragment of the 5'LTR
for high level transcription initiation
and retroviral genome synthesis. A modified packaging region was constructed
to add the wild-type MoMuLV splice acceptor
sequence. The addition of the splice acceptor increases mRNA stability and
the expression of the inserted gene.
Using the 293 gag-pol cell line retroviral
packaging system, high titers of viral particles
can be routinely obtained with all pRT-X-based
vectors. We provide the MoMuLV envelope gene expressed
from the CMV promoter/enhancer for high
level envelope expression and use in ecotropic
infections.
Retroviral particles containing an
ecotropic envelope are able to infect mouse
and rat, but not human cells.
For amphotropic envelope expression,
we provide the 4070A MuLV envelope gene
expressed from the CMV promoter/enhancer
vector.
Amphotropic envelope containing particles
can infect cells of most animal species
except hamsters.
pRT-X is the minimal retroviral
vector devoid of any internal gene sequences.
This vector may be used as a retroviral
expression vehicle where drug selection
to produce stable cell lines is not required.
For the pRT-X vector, we have inserted
the b-galactosidase or luciferase genes for use as
positive controls.
Ordering Information
| Products
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Contents |
Cat.No.
|
Price |
| pRT-X
Retroviral Kit |
pRT-X,
pRT-Z, pRT-L |
12007 |
350.00 |
| pRT-X
Neo Retroviral Kit |
pRT-X
Neo, pRT-Z Neo, pRT-L Neo & G418 |
12008 |
375.00 |
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