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Type of Sequencing Services

Back to Custom Sequencing

PRIMER EXTENSION SEQUENCING SERVICE Back to top

Result:	700-1100 bp sequence data per reaction.

Starting Material:	Provided templates from customer.
	Provided primers from customer
	Custom primers can be synthesized at our company for faster results.
	The following universal primers are available to you at no cost: T3, T7, Sp6, M13R, M13F, pGEX3', pGEX5', and BGH reverse.

Method:	Primer-extension sequencing by using big-dye chemistry or other related chemistry from Applied Biosystem.
	If the sequence data is unsatisfactory, the sequencing reaction is repeated with different conditions. The better result will be sent to the customer.

DIRECT PCR PRODUCT SEQUENCING SERVICE Back to Top

Result:	700-1100 bp sequence data per reaction.

Starting Material:	Purified PCR products from customer. Provided primers from customer.

Method:	Primer-extension by using big-dye chemistry or other related chemistry from Applied Biosystem.
	If the sequence data is unsatisfactory, the sequencing reaction is repeated with different conditions. The better result will be sent to the customer.

SINGLE STRANDED DNA SEQUENCING SERVICE Back to Top

Result:	Consensus sequence with > 99% accuracy.
	A complete report of sequencing strategies and data.

Starting Material:	Provided templates from customer.
	Customer-provided initial primers.
	Custom primers can be synthesized at our company for faster results.
	The following universal primers are available to you at no cost: T3, T7, Sp6, M13R, M13F, pGEX3', pGEX5', and BGH reverse.

Method:	Design and synthesize internal primers at the optimal interval for better overlap.
	Single stranded coverage of the template through multiple sequencing reactions.
	Assemble and edit the contigs?
	Resolve most problematic regions.
	The consensus sequence is generated with accuracy greater than 99 %.

DOUBLE STRANDED DNA SEQUENCING SERVICE Back to Top

Result:	Finished consensus sequence with greater than 99.99% accuracy.
	A complete report of sequencing strategies and data.

Starting Material:	Templates from customer.
	Customer-provided primers.
	Custom primers can be synthesized at our company for faster results.
	The following universal primers are available to you at no cost: T3, T7, Sp6, M13R, M13F, pGEX3', pGEX5', and BGH reverse.

Method:	Design and synthesize internal primers.
	Double stranded coverage of the template through multiple sequencing reactions.
	Assemble and edit the contigs?
	The problematic regions are completely determined.
	The consensus sequence is generated with accuracy greater than 99.99%.

BAC, PAC AND P1 ENDS SEQUENCING SERVICE Back to Top

Result	400-600 bp sequence data

Starting Material:	Provided templates form customer.
	Provided primers from customer

Method:	Proprietary protocols in conjunction with big-dye chemistry or other related chemistry from Applied Biosystems.
	If the sequence data is unsatisfactory, the sequencing reaction is repeated with different conditions. The better result will be sent to the customer.

cDNA/EST LIBRARY SEQUENCING SERVICE Back to Top

Result:	700-1100 bp sequence data per reaction

Starting Material:	Provided bacterial cultures or templates from customer.
	Customer-provided primers.
	Custom primers can be synthesized at our company for faster results.
	The following universal primers are available to you at no cost: T3, T7, Sp6, M13R, M13F, pGEX3', pGEX5', and BGH reverse.
	If the sequence data is unsatisfactory, the sequencing reaction is repeated with different condition. The better result will be sent to the customer.
Method:	Primer-extension by using big-dye kit or other related chemistry from Applied Biosystem.
	Use automated liquid sample handling instrument to prepare sequencing reactions.
	If the sequence data is unsatisfactory, the sequencing reaction is repeated with different conditions. The better result will be sent to the customer.
	The samples are cataloged and stored at -20 celcius degrees.

FDA SUBMISSION SEQUENCING SERVICE Back to Top

Result:	Finished consensus sequence with 100 % accuracy.
	A complete report of sequencing strategies and data.

Starting Material:	Templates from customer.
	Customer-provided primers.
	Custom primers can be synthesized at our company for faster results.
	The following universal primers are available to you at no cost: T3, T7, Sp6, M13R, M13F, pGEX3', pGEX5', and BGH reverse.

Method:	Use big-dye chemistry or other related chemistry from Applied Biosystem.
	Complete double stranded coverage of the template through 4 fold-redundancy sequencing.
	Resolve all problematic regions.
	Assemble and edit the final sequence.

PLASMID DNA PURIFICATION SERVICE Back to Top

Result:	Purified plasmids suitable for automated sequencing, transfection, and other molecular methodologies.

Starting Material:	Provided bacterial cultures or agar plates from customer.

Method:	Protocols from RETROGEN Ultrapure MiniPrep Kit.

PCR PRODUCT PURIFICATION SERVICE Back to Top

Result:	Purified PCR fragments are suitable for automated sequencing..

Starting Material:	Crude PCR products. Expected size of the PCR product to be isolated and purified

Method:	Protocols from RETROGEN Ultrapure MiniPrep Kit.

SHOTGUN LIBRARY CONSTRUCTION & SEQUENCING Back to Top

Result:	Original electropherograms
	Consensus sequence on diskette

Starting Material:	Shotgun sequencing strategies can be used to sequence a variety of samples including cosmids, BACs, PACs, and genomics.

Method:	Randomly generated plasmid clones using nebulization techniques.
	The random fragments (typically, 1-3 kb in length) are cloned into a standard vector with common restriction sites.
	Sequence data is obtained from each end of the random clones and assembled to provide up to 100% coverage of the entire clone.
	Contig alignment of each gel read

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Questions About Products Or Services, Call 1-800-RETROGEN Or 1-858-455-8411