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Call: 1-800-RETROGEN |
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Custom Mutagenesis |
In vitro site-directed mutagenesis is an important methodology used to study protein structure-function relationships, identify intramolecular regions or amino acids that may mediate these functions, analyze gene expression and modify vectors. Many published mutagenesis methodologies labor intensive and technically difficult to perform. With many years of experience in mutational manipulation, Retrogen has developed its own methodology that allows us to finish any mutagenesis project at very fast turnaround time and at a very affordable price. |
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The Benefits of Getting Mutagenesis Projects Done with Retrogen
- Get Consultation from Our Experienced Scientists
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- Get Your Mutagenesis Projects Done Fast at a Very Competitive Price
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- Sequence the Mutated Region to Ensure a 100% Accuracy
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- 20 ug of Purified Plasmid Delivered is Ready to Use
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- A Complete Report with Chromatogram Printout
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| Starting Material: |
20 ug of plasmid DNA provided from the customer.
Hard copies of vector map and sequence of the construct with indication of the bases that need to be changed |
Method: |
Using PCR site-directed method in conjunction with our proprietary protocols.
Sequence the mutated region by automated fluorescent sequencing |
Final Form : |
A 20 ug of lyophilized plasmid DNA with the correct changes |
Documentation:
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A complete report of strategies and data
A printout of assembly report and chromatograms from automated sequencers |
Price
The cost for each mutant is 700 dollars which includes both strands sequencing verification. Please call us for a discounted price if you have more than one mutant to be made. |
Turnaround time
Each mutant will take 2-3 weeks, including sequence verification. |
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Ordering
Make sure that the correct changes are indicated on the sequence printout of the construct
Copy modified sequence and original sequence to the diskette
Enclose 20 ug plasmid DNA, vector map and a diskette in a shipping envelope.
Send the package priority overnight to the following address:
Retrogen, Inc
Gene Construction Department
6645 Nancy Ridge Drive
San Diego, CA 92121
858-455-8411 (Tel)
858-455-7987 (Fax)
info@retrogen.com (E-mail) |
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