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The
availability of sequences of entire genomes
has dramatically increased the number of
protein targets, many of which will need
to be overexpressed in cells other than
the original source of DNA. Gene synthesis
often provides a fast and economically efficient
approach. The synthetic gene can be optimized
for expression and constructed for easy
mutational manipulation without regard to
the parent genome. Yet design and construction
of synthetic genes, especially those coding
for large proteins, can be a slow, difficult
and confusing process. Through many years
of experience in synthetic gene construction,
Retrogen has developed a proprietary system
by integrating software, instrumentation
and protocols that allows us to complete
any gene size at a very rapid turnaround
time. With this proprietary system Retrogen
can offer you complete gene synthesis service,
from optimizing the codon to confirming
the sequence of the synthetic gene at a
very competitive price.
The Advantages
of Ordering Custom Gene with Retrogen
Optimized
gene sequences
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Design
and synthesize all primers
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Minimize
RNA secondary structures
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Add
and/or remove restriction sites
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Guarantee
full sequence verification of both
strands
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Fast
Turnaround times
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| Specifications
for Custom Gene Synthesis |
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Minimum
Sequence Length: |
100 base pairs
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Maximum
Sequence Length: |
15,000
base pairs |
| Gene
Optimization Options: |
Codon
preference for host organism, secondary
structure removal, GC content adjustment,
addition or removal of restrictions
sites |
| Starting
Material: |
A
sequence of interest gene in a diskette
or email it to us. |
| Gene
Construction : |
Using
proprietary gene synthesis software
to design and construct the entire
gene.
Clone
the synthetic gene in the standard
plasmid |
| DNA
Sequencing: |
Sequence
both strands to verify the integrity
of the synthetic gene sequence. |
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Final
Form of Construct: |
20
ug of lyophilized plasmid DNA containing
the synthetic gene.
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| Results: |
A
complete report of gene construction
A
plasmid map and printout of
assembly report and chromatograms
from automated sequencers
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Gene
Synthesis Prices
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Size of Gene |
Price |
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50 bp - 435 bp |
$1000/gene flat rate |
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436 bp - 1000 bp |
$2.30/bp |
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1001 bp - 1800 bp |
$2.75/bp |
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1801 bp - 3000 bp |
$3.00/bp |
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>3000 bp |
inquiry |
Turnaround
Time
It
will take 2-3 weeks per kb.
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Ordering
Make sure the final gene sequence
optimized and modified to your own
specifications
Obtain a price quote from Retrogen
custom gene synthesis department at
gene@retrogen.com
Email gene sequences and PO
number to gene@retrogen.com
or send to the following address:
Retrogen,
Inc
Gene Construction Department
6645 Nancy Ridge Drive
San Diego, CA 92121
858-455-8411 (Tel)
858-455-7987 (Fax)
info@retrogen.com
(E-mail)
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