Description
The PCR Purification system is designed for fast cleanup of DNA fragments from enzymatic PCR reactions. The Retrogen PCR Purification columns contain a silica-gel membrane for binding of up to 10 µg DNA in high-salt buffer and elution in low-salt buffer. Impurities are washed away, and pure DNA is eluted in a small volume of water, ready for use in any subsequent application. The purification procedure removes nucleotides, primers, enzymes, mineral oil, salts, detergents and other impurities from DNA samples. Retrogen silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Binding buffers promote selective adsorption of DNA molecules within the size range of 70 bp to 10 kb.
Key Benefits
Efficient removal of contaminants
Up to 95% recovery of ready-to-use DNA
Generate high quality of automated sequencing data
DNA cleanup in 3 simple steps: just bind, wash, and elute
No resins, no phenol, no alcohol precipitation
Get quality automated squencing data from RetroPrep PCR purifidation kit
QC analysis of RetroPrep PCR Purification kit. The sequence data was generated from ABI 3730 DNA sequencer, using Bigdye terminator kit from Applied Biosystems.
| Product |
Contents |
Cat.
No. |
Price |
Order |
RetroPrep PCR
Purification (100) |
100 RetroPrep spin columns |
14001 |
100.00 |
|
RetroPrep PCR
Purification (250) |
250 RetroPrep spin columns |
14002 |
225.00 |
|
|
