|
|
| |
|
| |
|
| |
| To
Receive 2 FREE
Sequencing or Oligo Services Sign
in |
|
|
|
|
|
|
|
|
|
|
|
 |
| |
|
|
| |
| |
---W
e l c o m e t o R e t r o g e n--- |
| |
|
| |
New
Customers Receive
2
Free Reactions on the First Sequencing
Order! |
| |
|
| |
|
| |
|
|
Next
Generation of ABI 3730 Capillary Sequencers
Call 1-800-RETROGEN
|
| |
|
| |
| Retrogen
Custom siRNA Construction Services |
Gene silencing of target mRNA by RNA interference
(RNAi) has dramatically enhanced the arsenal of
genetic tools that can be used to study gene function.
RNAi is a process used by plants and invertebrates
to specifically inactivate gene function. It has
been shown that double-stranded RNA (dsRNA) complementary
to particular messenger RNA (mRNA) can specifically
inactivate gene function through the RNAi pathway
in mammalian systems as well. Small interfering
RNAs (siRNAs) are generated in vivo from long precursor
molecules through the action of a specific RNAase
III endonuclease called Dicer. Dicer initiates the
cleavage of the precursor into ~22 nucleotide (nt)
double-stranded duplexes with two nt 3'-overhangs
and 5' phosphate termini termed short interfering
RNAs (siRNAs). The siRNA is subsequently incorporated
into the RNA induced silencing complex (RISC), a
protein/RNA complex. The siRNA allows specific recognition
of the mRNA target and the endonuclease function
of the RISC results in cleavage of mRNA and thus
loss of gene expression.
Retrogen
staff with experience in gene inhibition can provide
you a complete siRNA service and to help you speed-up
your gene silencing experiments. With your gene
reference sequence number or your siRNA target of
interest, we will perform all necessary steps, from
choosing the siRNA target sequence on your gene
to validating the resulting siRNA insert.
|
|
|
|
